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goat polyclonal anti-mouse chitinase 3-like 3/ecf-l antibody  (R&D Systems)


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    R&D Systems goat polyclonal anti-mouse chitinase 3-like 3/ecf-l antibody
    Goat Polyclonal Anti Mouse Chitinase 3 Like 3/Ecf L Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti-mouse chitinase 3-like 3/ecf-l antibody/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    goat polyclonal anti-mouse chitinase 3-like 3/ecf-l antibody - by Bioz Stars, 2026-05
    90/100 stars

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    Western blot analysis of the expressions of collagen type I, IRF-5 (M1), IL-12 (M1), and YM-1 <t>(M2)</t> in normal and fibrotic mouse (A) and human (B) livers . * p < 0.05. N = 5 cirrhotic human livers, N = 6 normal human livers, N = 4 normal mouse livers, N = 6 fibrotic mouse livers.
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    Western blot analysis of the expressions of collagen type I, IRF-5 (M1), IL-12 (M1), and YM-1 (M2) in normal and fibrotic mouse (A) and human (B) livers . * p < 0.05. N = 5 cirrhotic human livers, N = 6 normal human livers, N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

    Journal: Frontiers in Immunology

    Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

    doi: 10.3389/fimmu.2014.00430

    Figure Lengend Snippet: Western blot analysis of the expressions of collagen type I, IRF-5 (M1), IL-12 (M1), and YM-1 (M2) in normal and fibrotic mouse (A) and human (B) livers . * p < 0.05. N = 5 cirrhotic human livers, N = 6 normal human livers, N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

    Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

    Techniques: Western Blot

    Localization TGM-2 (M2) in human livers . (A,B) Immunohistochemical localization of TGM-2 in normal (A) and cirrhotic (B) human livers. Note the presence of the strongly stained cells in the fibrotic matrix (F) . (C) Co-localization of TGM-2 (blue staining) and CD68 (red staining). (D) Co-localization of TGM-2 (blue staining) and CD206 (red staining). Arrows indicate co-localization. Magnifications: 100× (A,B) and 400× (C,D) . N = 5 cirrhotic human livers, N = 6 normal human livers.

    Journal: Frontiers in Immunology

    Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

    doi: 10.3389/fimmu.2014.00430

    Figure Lengend Snippet: Localization TGM-2 (M2) in human livers . (A,B) Immunohistochemical localization of TGM-2 in normal (A) and cirrhotic (B) human livers. Note the presence of the strongly stained cells in the fibrotic matrix (F) . (C) Co-localization of TGM-2 (blue staining) and CD68 (red staining). (D) Co-localization of TGM-2 (blue staining) and CD206 (red staining). Arrows indicate co-localization. Magnifications: 100× (A,B) and 400× (C,D) . N = 5 cirrhotic human livers, N = 6 normal human livers.

    Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

    Techniques: Immunohistochemical staining, Staining

    Localization of YM-1 (M2) in mouse livers . (A) Co-localization of YM-1 (red staining) and CD68 (blue staining). (B) Co-localization of YM-1 (red staining) and CD206 (blue staining). (C–F) Immunohistochemical localization of YM-1 in livers of normal mice (C,E) and in advanced fibrosis (D,F) . Magnifications: 40× (C,D) , 200× (E,F) , and 400× (A,B) . N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

    Journal: Frontiers in Immunology

    Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

    doi: 10.3389/fimmu.2014.00430

    Figure Lengend Snippet: Localization of YM-1 (M2) in mouse livers . (A) Co-localization of YM-1 (red staining) and CD68 (blue staining). (B) Co-localization of YM-1 (red staining) and CD206 (blue staining). (C–F) Immunohistochemical localization of YM-1 in livers of normal mice (C,E) and in advanced fibrosis (D,F) . Magnifications: 40× (C,D) , 200× (E,F) , and 400× (A,B) . N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

    Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

    Techniques: Staining, Immunohistochemical staining

    Expressions of YM-1 (M2) in fibrotic mouse livers [4 weeks CCl4 (A,B)] and in fibrotic livers undergoing resolution [after cessation of CCl4 administration (C,D)] . Immunohistochemical pictures demonstrate an overview [ (A,C) magnification 40×] and close up [ (B,D) magnification 200×]. (E) Western blot quantification of YM-1 expression in fibrosis versus resolution. ** p < 0.01. N = 6/group.

    Journal: Frontiers in Immunology

    Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

    doi: 10.3389/fimmu.2014.00430

    Figure Lengend Snippet: Expressions of YM-1 (M2) in fibrotic mouse livers [4 weeks CCl4 (A,B)] and in fibrotic livers undergoing resolution [after cessation of CCl4 administration (C,D)] . Immunohistochemical pictures demonstrate an overview [ (A,C) magnification 40×] and close up [ (B,D) magnification 200×]. (E) Western blot quantification of YM-1 expression in fibrosis versus resolution. ** p < 0.01. N = 6/group.

    Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

    Techniques: Immunohistochemical staining, Western Blot, Expressing